CD38 Antibody, anti-human

Name: CD38 Antibody, anti-human
Availability: InStock

CD38 Antibody, anti-humanExtended Validation

Clone:

IB6

Type of antibody:

Primary antibodies

Isotype:

mouse IgG2aκ

Applications:

FC, MICS, IF, IHC, ICC

Alternative names:

ADPRC 1, ADPRC1, T10

Flow cytometry applications forCD38 Antibody, anti-human

APC
Biotin
FITC
PE
PE-Vio 770
Vio Bright B515

Conjugate:

APC

Recommended dilution:

1:50

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Reported:

20372106, 28402853,

Products used:

130-113-986, 130-113-424

Protocols:

Cell surface flow cytometry staining protocol (PBS/EDTA/BSA 1:50)

Description:

Human peripheral blood mononuclear cells (PBMCs) were stained with CD38 antibodies or with the corresponding isotype control antibodies (left images) as well as with CD19 antibodies and analyzed by flow cytometry using the MACSQuant^® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD38 Antibody, anti-human

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

PFA

Tested:

during development

Species tested:

human

Products used:

130-113-986, 130-113-424

Compatible applications:

IF, IHC, ICC

Protocols:

Protocol for MICS experiments

Conjugate:

APC

Recommended dilution:

1:50

Fixation:

Acetone

Tested:

during development

Species tested:

human

Products used:

130-113-986, 130-113-424

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Extended validation for CD38 Antibody, anti-human

Specificity

Knockout validation

To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.

WT

KO

Fluorescence microscopy image of CD38 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD38-PE (IB6, red) and counterstained with DRAQ5 (blue) as DNA stain.

Fluorescence microscopy image of CD38 knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD38-PE (IB6, red) and counterstained with DRAQ5 (blue) as DNA stain.

Overlay histogram showing flow cytometric analysis of CD38 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD38-PE, clone (IB6). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Overlay histogram showing flow cytometric analysis of CD38 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD38-PE, clone (IB6). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD38. Human peripheral blood mononuclear cells (PBMCs) were stained with CD38antibodies and with a suitable counterstaining. As a control, CD38 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD38 (IB6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD38 (IB6). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD38 Antibody, anti-human

Overview

Clone IB6 recognizes the human CD38 antigen, a single-chain type II transmembrane glycoprotein with enzymatic activity. It is present on the majority of hematopoietic cells, prevalent during early differentiation and activation processes. Terminally differentiated B cells (plasma cells) express CD38 brightly. Furthermore, CD38 is constitutively expressed in several tissues, for example brain, muscle, and kidney.

Alternative names

ADPRC 1, ADPRC1, T10

Detailed product information

Technical specifications

Clone	IB6
Clonality	monoclonal
Isotype	mouse IgG2aκ
Isotype control	Isotype Control Antibody, mouse IgG2a
Host	mouse
Type of antibody	Primary antibodies
Species	human
Antigen	CD38
Alternative names of antigen	ADPRC 1, ADPRC1, T10
Molecular mass of antigen [kDa]	34
Distribution of antigen	B cells, leukocytes, lymphocytes, monocytes, myeloid leukemia cells, NK cells, red blood cells, T cells, basophils, plasma cells, thymocytes, bone marrow, brain, kidney, ovary, pancreas, placenta, skeletal muscle
Entrez Gene ID	952
RRID	AB_2733640, AB_2733812, AB_2733813, AB_2733374, AB_2733375, AB_2733229, AB_2733230, AB_2733520, AB_2733521, AB_2922075, AB_2922040, AB_2733639

Resources for CD38 Antibody, anti-human

Certificates

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References for CD38 Antibody, anti-human

Publications

Yoon-Sang, K. et al. (2010) Transduction of human primitive repopulating hematopoietic cells with lentiviral vectors pseudotyped with various envelope proteins. Mol. Ther. 18(7): 1310-1317

Applications

Extended validation