Name: CD34 MultiSort Kit, human
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Product data and images for CD34 MultiSort Kit, human

Figures

Figure 1

CD34

⁺

HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34

⁺

cells from PBMCs was followed by the depletion of CD34

⁺

HLA-DR

⁺

cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34

⁺

HLA-DR

^–

cells (red).

A:	B:
PBMCs before separation	CD34 ⁺ cells
View details CD34 MultiSort Kit, human Figure 1 CD34 ⁺ HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34 ⁺ cells from PBMCs was followed by the depletion of CD34 ⁺ HLA-DR ⁺ cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34 ⁺ HLA-DR ^– cells (red).	View details CD34 MultiSort Kit, human Figure 1 CD34 ⁺ HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34 ⁺ cells from PBMCs was followed by the depletion of CD34 ⁺ HLA-DR ⁺ cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34 ⁺ HLA-DR ^– cells (red).
C:
CD34 ⁺ HLA-DR ^- cells
View details CD34 MultiSort Kit, human Figure 1 CD34 ⁺ HLA-DR– cells isolated from human PBMCs using the CD34 MultiSort Kit. Positive selection of CD34 ⁺ cells from PBMCs was followed by the depletion of CD34 ⁺ HLA-DR ⁺ cells using HLA-DR-FITC and Anti-FITC MicroBeads resulting in a population of CD34 ⁺ HLA-DR ^– cells (red).

Figure 2

Step-by-step CD34 MultiSort separation procedure.

Figure 2

Step-by-step CD34 MultiSort separation procedure.

Figure 3

CD34
⁺
lin
^-
cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)

Figure 3

CD34

⁺

lin

cells isolated from peripheral blood using CD34 MultiSort Kit, May-Grünwald-Giemsa stained. (Courtesy of Dr. Ziegler, Tübingen, Germany.)

Specifications for CD34 MultiSort Kit, human

Overview

The CD34 MultiSort Kit has been developed for the positive selection of CD34

⁺

subpopulations by sequential sorting. CD34

⁺

hematopoietic stem and progenitor cells can be isolated from

peripheral blood,
bone marrow aspirate,
leukapheresis product,
cord blood,
differentiated ES and iPS cell cultures¹.

Detailed product information

Detailed separation procedure

Using the CD34 MultiSort Kit, in the first step CD34

⁺

cells are positively selected after labeling with the CD34 MultiSort MicroBeads. Subsequently, the MultiSort MicroBeads are enzymatically released from the CD34 antibody. The isolated CD34

⁺

cells can be magnetically labeled and sorted according to a second antigen. The kit contains CD34 MultiSort MicroBeads, MultiSort Release Reagent, and MultiSort Stop Reagent.

Control staining after separation requires a monoclonal CD34 antibody other than QBEND/10, e.g. clone AC136

Downstream applications

The CD34 MultiSort Kit is used to isolate specific subsets of CD34

⁺

hematopoietic stem and progenitor cells. CD34-selected cells can be further sorted according to their expression of, e.g., HLA-DR, CD43, CD45RA, CD61, CD71, CD90 (Thy1), or CD133.

Columns

MS, LS, XS, or autoMACS

^®

Columns.

References for CD34 MultiSort Kit, human

Publications

Vodoyanik, M. A. et al. (2006) Leukosialin (CD43) defines hematopoietic progenitors in human embryonic stem cell differentiation cultures. Blood 108: 2095-2105

Data and images