CD34 MicroBead Kit, human

Name: CD34 MicroBead Kit, human
Availability: InStock

CD34 MicroBead Kit, human

The CD34 MicroBead Kit has been developed for the positive selection of CD34

⁺

hematopoietic stem and progenitor cells in a single step. CD34

⁺

cells can be isolated from:

peripheral blood (PBMCs)
bone marrow¹
leukapheresis product²
cord blood³
differentiated ES and iPS cell lines^4,5.

Product data and images for CD34 MicroBead Kit, human

Figures

Figure 1

Flow cytometric analysis of CD34

⁺

cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.

BM-MNCs before separation	Isolated CD34 ⁺ cells
View details CD34 MicroBead Kit, human Figure 1 Flow cytometric analysis of CD34 ⁺ cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.	View details CD34 MicroBead Kit, human Figure 1 Flow cytometric analysis of CD34 ⁺ cells isolated from bone marrow mononuclear cells (BM-MNCs) using the CD34 MicroBead Kit, an MS Column, and a MiniMACS™ Separator.

Specifications for CD34 MicroBead Kit, human

Overview

The CD34 MicroBead Kit has been developed for the positive selection of CD34

⁺

hematopoietic stem and progenitor cells in a single step. CD34

⁺

cells can be isolated from:

peripheral blood (PBMCs)
bone marrow¹
leukapheresis product²
cord blood³
differentiated ES and iPS cell lines^4,5.

Detailed product information

Background information

CD34 is a well-established marker of human hematopoietic stem and progenitor cells and additionally expressed on hemangioblasts

^5,6

, endothelial progenitor cells

^5,7-9

, and mature endothelial cells

⁸

Control staining of cells isolated with the CD34 MicroBead Kit requires a monoclonal CD34 antibody other than QBEND/10, e.g., clone AC136.

Downstream applications

Cells isolated with the CD34 MicroBead Kit are suitable for various downstream applications, including:

flow cytometric analysis
expansion in culture
in vitro differentiation⁵
reprogramming³
retroviral transduction¹⁰
gene expression analyses
transplantation into humanized mice

Columns

MS, LS, XS, or autoMACS

^®

Columns.

Resources for CD34 MicroBead Kit, human

Documents and Protocols

Brochures/posters

Solutions for cutting-edge human hematopoietic stem cell research

App notes/customer reports

Array-based analysis of microRNA and mRNA expression characterizes early differentiation pathways of CD133⁺ and CD34⁺ hematopoietic stem and progenitor cells.

App notes/customer reports

Generation and preclinical applications of humanized mouse models to study human immunity and cancer immunotherapy

Reviews for CD34 MicroBead Kit, human

CD34 Microbeads Review

CD34 MicroBead Kit, human (130-046-702)

We are interested in enhancing the killing capacity of HIV-specific CD8 T cells by reengineering TCRs expressed by these cells. One of the approaches to do so, is to reprogram T cells at the stem cell level. As such, we use the CD34 Microbeads for stem cells purification from leukopacks.

Beads For Isolation Of Hematopoietic Stem Cells From PBMCs

CD34 MicroBead Kit, human (130-046-702)

Our lab has developed different mouse models to study the efficacy of therapeutic candidates against HIV. One of the models we have generated consists of injecting human hematopoietic stem cells into immunocompromised NSG mice. We later infect these mice with HIV to study different aspects of infection in vivo. We use this kit to purify human hematopoietic stem cells from blood.

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References for CD34 MicroBead Kit, human

Publications

Brault, J. et al. (2014)
Optimized generation of functional neutrophils and macrophages from patient-specific induced pluripotent stem cells:
ex vivo
models of X
⁰
-linked, AR22
⁰
- and AR47
⁰
- chronic granulomatous diseases.
Biores Open Access 3(6): 311-326
Schreiber, T. D. et al. (2009) The integrin alpha9beta1 on hematopoietic stem and progenitor cells: involvement in cell adhesion, proliferation and differentiation. Haematologica 94: 1493-1501
Jing, D. et al. (2010) CD49d blockade by natalizumab in patients with multiple sclerosis affects steady-state hematopoiesis and mobilizes progenitors with a distinct phenotype and function. Bone Marrow Transplant. 45: 1489-1496
Ban, H. et al. (2011) Efficient generation of transgene-free human induced pluripotent stem cells (iPSCs) by temperature-sensitive Sendai virus vectors. Proc. Natl. Acad. Sci. U.S.A. 108: 14234-9
Bandi, S. and Akkina, R. (2008) Human embryonic stem cell (hES) derived dendritic cells are functionally normal and are susceptible to HIV-1 infection. AIDS Res. Ther. 5: 1
Wang, Z. Z. et al. (2007) Endothelial cells derived from human embryonic stem cells form durable blood vessels in vivo. Nat. Biotechnol. 25: 317-318
Loges, S. et al. (2004) Identification of the adult human hemangioblast. Stem Cells Dev. 13: 229-242
Peichev, M. et al. (2000)
Expression of VEGFR-2 and AC133 by circulating human CD34
⁺
cells identifies a population of functional endothelial precursors.
Blood 95: 952-958
Rafii, S. and Lyden, D. (2003) Therapeutic stem and progenitor cell transplantation for organ vascularization and regeneration. Nat Med 9: 702-712
Valgimigli, M. et al. (2004)
CD34
⁺
and endothelial progenitor cells in patients with various degrees of congestive heart failure.
Circulation 110: 1209-1212
Chen, T. H. P. et al. (2011) Knockdown of Hspa9, a del(5q31.2) gene, results in a decrease in hematopoietic progenitors in mice. Blood 117: 1530-1539

Data and images