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Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant ® Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence. |
CD33 antibodiesFigure 1Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant ® Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence. | CD33 antibodiesFigure 1Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant ® Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence. |
Clone | AC104.3E3 |
---|---|
Isotype | mouse IgG1 |
Type of antibody | Primary antibodies |
Species | human |
Antigen | CD33 |
Alternative names of antigen | My9, p67, Siglec-3 |
Molecular mass of antigen [kDa] | 38 |
Distribution of antigen | dendritic cells, granulocytes, Langerhans cells, macrophages, mast cells, monocytes, NK cells, T cells |
Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant ® Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence. |
CD33 antibodiesFigure 1Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant ® Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence. | CD33 antibodiesFigure 1Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant ® Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence. |
Clone | AC104.3E3 |
---|---|
Isotype | mouse IgG1 |
Type of antibody | Primary antibodies |
Species | human |
Antigen | CD33 |
Alternative names of antigen | My9, p67, Siglec-3 |
Molecular mass of antigen [kDa] | 38 |
Distribution of antigen | dendritic cells, granulocytes, Langerhans cells, macrophages, mast cells, monocytes, NK cells, T cells |
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