CD33 antibodies
Clone:
AC104.3E3
Type of antibody:
Primary antibodies
Isotype:
mouse IgG1
Alternative names:
My9, p67, Siglec-3

Data and images for CD33 antibodies

Figures

Figure 1

Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant
®
Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.
View details

Figure 1

Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant
®
Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.
View details

Figure 1

Human EDTA-treated whole blood samples were stained with CD33 antibodies as well as CD14 antibodies using a lyse-wash procedure and analyzed by flow cytometry using the MACSQuant
®
Analyzer. For analysis, mononuclear cells were gated based on forward and side scatter signals and after exclusion of cell debris and red blood cells based on scatter signals and CD45 fluorescence.

Specifications for CD33 antibodies

Overview

AC104.3E3 recognizes the human CD33 antigen, a 67 kDa glycoprotein belonging to the sialoadhesin superfamily. The CD33 antigen is highly expressed on human monocytes but weakly on granulocytes and some – but not all – myeloid dendritic cells. The CD33 antigen is also found on myeloid progenitor cells (CFU-GEMM, CFU GM, CFU-G, BFU-E) but is not expressed on lymphocytes, platelets, erythrocytes, or primitive hematopoietic stem cells.

Applications

Reagent can be used for immunophenotyping by flow cytometry. Abnormal numbers of cells expressing this antigen or aberrant expression levels of the antigen can be expected in some disease states. It is important to understand the normal expression pattern for this antigen and its relationship to expression of other relevant antigens in order to perform appropriate analysis.
Expression of CD33 may be used as aid to diagnostic in the characterization of samples from individuals suspected with hematologic neoplasia.

Alternative names

My9, p67, Siglec-3

Detailed product information

Technical specifications

CloneAC104.3E3
Isotypemouse IgG1
Type of antibodyPrimary antibodies
Specieshuman
AntigenCD33
Alternative names of antigenMy9, p67, Siglec-3
Molecular mass of antigen [kDa]38
Distribution of antigendendritic cells, granulocytes, Langerhans cells, macrophages, mast cells, monocytes, NK cells, T cells

Resources for CD33 antibodies

Certificates

Please follow this
link
to download the Certificate of Conformity (CoC) by lot number.

References for CD33 antibodies

Publications

  1. Bernstein, I. D. et al. (1987)
    Treatment of acute myeloid leukemia cells
    in vitro
    with a monoclonal antibody recognizing a myeloid differentiation antigen allows normal progenitor cells to be expressed.
    J. Clin. Invest. 79(4): 1153-1159
  2. Stelzer, G. T. et al. (1997) U.S.-Canadian consensus recommendations on the immunophenotypic analysis of hematologic neoplasia by flow cytometry: standardization and validation of laboratory procedures. Cytometry 30(5): 214-230
  3. Rothe, G et al. (2012) Consensus protocol for the flow cytometric Immunophenotyping of hematopoietic malignancies. Leukemia 10(5): 877-895
  4. van Dongen, J. J. M. et al. (2012) EuroFlow antibody panels for standardized n-dimensional flow cytometric immunophenotyping of normal, reactive and malignant leukocytes. Leukemia 26(9): 1908-1975
  5. Clinical and Laboratory Standards Institute (CLSI) (2007) Clinical Flow Cytometric Analysis of Neoplastic Hematolympoid Cells, CLSI document H43-A2 (ISBN 1-56238-635-2) CLSI; Approved Guideline - Second Edition
  6. Clinical and Laboratory Standards Institute (CLSI) (2007) Enumeration of immunologically defined cell populations by flow cytometry, CLSI document H42-A2 (ISBN 1-56238-640-9) CLSI; Approved Guideline - Second Edition
  7. Wood, B. L. et al. (2007) 2006 Bethesda International Consensus recommendations on the immunophenotypic analysis of hematolymphoid neoplasia by flow cytometry: optimal reagents and reporting for the flow cytometric diagnosis of hematopoietic neoplasia. Cytometry B Clin. Cytom. 72: 14-22

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