CD321 (JAM1) Antibody, anti-human, REAfinity™

Name: CD321 (JAM1) Antibody, anti-human, REAfinity™
Availability: InStock

CD321 (JAM1) Antibody, anti-human, REAfinity™Extended ValidationRecombinant

Clone:

REA605

Type of antibody:

Primary antibodies, Recombinant antibodies

Isotype:

recombinant human IgG1

Applications:

FC, MICS, IF, IHC

Alternative names:

JAM-1, JAM-A, PAM-1, F11 R

Flow cytometry applications forCD321 (JAM1) Antibody, anti-human, REAfinity™

APC
APC-Vio 770
Biotin
FITC
PE
PE-Vio 770
PerCP-Vio 700
VioBlue

Conjugate:

APC

Recommended dilution:

1:11

Remark:

Cells should be stained prior to fixation, if formaldehyde is used as a fixative.

Tested:

QC tested

Products used:

130-109-483, 130-109-404

Protocols:

Flow cytometry staining protocol for CD321 antibodies (1:11)

Description:

Human platelets were stained with CD321 (JAM1) antibodies or with the corresponding REA Control (S) antibodies (left image) as well as with CD61 antibodies. Flow cytometry was performed using the MACSQuant^® Analyzer. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.

MICS (MACSima Imaging Cyclic Staining) applications forCD321 (JAM1) Antibody, anti-human, REAfinity™

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:11

Fixation:

PFA

Tested:

during development

Species tested:

human

Products used:

130-109-483, 130-109-404

Compatible applications:

IF, IHC

Protocols:

Protocol for MICS experiments

Extended validation for CD321 (JAM1) Antibody, anti-human, REAfinity™

Specificity

Knockout validation

To ensure antibody specificity, the target gene is knocked out in a suitable cell line using the CRISPR/Cas9 system and the knockout is confirmed by sequencing of the target locus. The antibody is considered to bind specifically to the intended epitope if no antibody binding to the knockout cells can be detected. The antibody staining is controlled by fluorescence microscopy and/or flow cytometry.

WT

KO

Fluorescence microscopy image of CD321 (JAM1) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD321 (JAM1)-PE (REA605, red) and counterstained with DRAQ5 (blue) as DNA stain.

Fluorescence microscopy image of CD321 (JAM1) knockout cells. Wild type (WT, left) and knockout cells (KO, right) were stained with CD321 (JAM1)-PE (REA605, red) and counterstained with DRAQ5 (blue) as DNA stain.

Overlay histogram showing flow cytometric analysis of CD321 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD321-PE, clone (REA605). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Overlay histogram showing flow cytometric analysis of CD321 knockout cells. Wild type (red) and knockout cells (blue) were stained with CD321-PE, clone (REA605). Flow cytometry was performed with the MACSQuant

^®

Analyzer. Cell debris, dead cells and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for CD321 (JAM1) Antibody, anti-human, REAfinity™

Overview

Clone REA605 recognizes the human CD321 antigen, a cell-surface adhesion molecule, also known as JAM1 or JAMA. CD321 is a type I transmembrane glycoprotein belonging to the immunoglobin superfamily. It has two extracellular Ig-like domains, a transmembrane region and a short cytosolic tail.

CD321 is expressed on a variety of cell types: erythrocytes, leukocytes, platelets, as well as endothelial and epithelial cells. CD321 has different functions. It is a regulator of tight junction assembly in epithelia and acts as a receptor for reovirus. Furthermore CD321 binds integrin LFA1 and is involved in leukocyte transmigration. In addition, CD321 is a platelet receptor.

Additional information: Clone REA605 displays negligible binding to Fc receptors.

Alternative names

JAM-1, JAM-A, PAM-1, F11 R

Detailed product information

Technical specifications

Clone	REA605
Clonality	monoclonal
Isotype	recombinant human IgG1
Isotype control	REA Control Antibody (S), human IgG1
Host	human cell line
Type of antibody	Primary antibodies, Recombinant antibodies
Species	human
Antigen	CD321 (JAM1)
Alternative names of antigen	JAM-1, JAM-A, PAM-1, F11 R
Molecular mass of antigen [kDa]	30
Distribution of antigen	endothelial cells, epithelial cells, platelets, red blood cells, leukocytes
Entrez Gene ID	50848
RRID	AB_2904942, AB_2657454, AB_2657455, AB_2657456, AB_2657457, AB_2657458, AB_2657459, AB_2657460, AB_2657461, AB_2657462, AB_2657463, AB_2657464, AB_2657465, AB_2657466, AB_2657467, AB_2904943

Resources for CD321 (JAM1) Antibody, anti-human, REAfinity™

Certificates

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References for CD321 (JAM1) Antibody, anti-human, REAfinity™

Publications

Fraemohs, L. et al. (2004) The functional interaction of the beta 2 integrin lymphocyte function-associated antigen-1 with junctional adhesion molecule-A is mediated by the I domain. J Immunol 173(10): 6259-6264
Ozaki, H. et al. (1999) Cutting edge: combined treatment of TNF-alpha and IFN-gamma causes redistribution of junctional adhesion molecule in human endothelial cells. J Immunol 163(2): 553-557
Sobocka, M. B. et al. (2000) Cloning of the human platelet F11 receptor: a cell adhesion molecule member of the immunoglobulin superfamily involved in platelet aggregation. Blood 95(8): 2600-2609

Applications

Extended validation