Clone:
REA736
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
HTA1, CD1, FCB6, R4, T6, Leu-6

Extended validation for CD1a Antibody, anti-human, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA736
HI149++
NA1/34-HLK-
SK9-
Cells were incubated with an excess of purified unconjugated CD1a (REA736) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD1a. A mixture of Human peripheral blood mononuclear cells (PBMCs) and MOLT4 cells were stained with CD1a antibodies and with a suitable counterstaining. As a control, CD1a antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD1a (REA736). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD1a (REA736). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD1a (REA736). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD1a (REA736). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD1a (REA736). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD1a Antibody, anti-human, REAfinity™

Overview

Clone REA736 recognizes the human CD1a antigen, a 40 kDa type I membrane glycoprotein, also known as T6 or Leu-6, which is a member of the immunglobulin superfamily. It shares functional and structural similarities with MHC I class molecules and is associated with β2-microglobulin. CD1a plays a role in antigen presentation by binding lipid and glycolipid antigens and presenting them to T cell receptors on natural killer T cells. CD1a is expressed on Langerhans cells, dendritic cells, and cortical thymocytes.
Additional information: Clone REA736 displays negligible binding to Fc receptors

Alternative names

HTA1, CD1, FCB6, R4, T6, Leu-6

Detailed product information

Technical specifications

CloneREA736
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD1a
Alternative names of antigenHTA1, CD1, FCB6, R4, T6, Leu-6
Molecular mass of antigen [kDa]35
Distribution of antigenB cells, dendritic cells, Langerhans cells, macrophages, monocytes, lymphocytes, thymocytes
Entrez Gene ID909
RRIDAB_2656004, AB_2656005, AB_2656006, AB_2656007, AB_2656008, AB_2656009, AB_2656010, AB_2656011, AB_2656012, AB_2656013, AB_2656014, AB_2656015, AB_2656016, AB_2656017, AB_2656018, AB_2889493, AB_2889492, AB_2889495, AB_2889494, AB_2656003

Resources for CD1a Antibody, anti-human, REAfinity™

Certificates

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References for CD1a Antibody, anti-human, REAfinity™

Publications

  1. Hanau, D. et al. (1990) Possible mechanism of action of CD1a antigens. J. Invest. Dermatol. 95(5): 503-505
  2. Calabi, F. et al. (1991) The CD1 system. Tissue Antigens 37(1): 1-9
  3. Blumberg, R. S. et al. (1995) Structure and function of the CD1 family of MHC-like cell surface proteins. Immunol. Rev. 147: 5-29

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