Clone:
REA265
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC, MICS, IF, IHC
Alternative names:
GP27, MER2, PETA-3, RAPH, SFA1, TSPAN24

Extended validation for CD151 Antibody, anti-human, REAfinity™

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD151. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD151 antibodies and with a suitable counterstaining. As a control, CD151 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD151. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD151 antibodies and with a suitable counterstaining. As a control, CD151 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD151. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD151 antibodies and with a suitable counterstaining. As a control, CD151 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD151. Human peripheral blood mononuclear cells (PBMCs) after erythrocyte lysis were stained with CD151 antibodies and with a suitable counterstaining. As a control, CD151 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and propidium iodide fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD151 (REA265). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD151 (REA265). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD151 (REA265). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD151 Antibody, anti-human, REAfinity™

Overview

Clone REA265 recognizes the CD151 antigen, a 29 kDa cell surface glycoprotein, which is also known as platelet-endothelial tetraspan antigen 3 (PETA-3) or tetraspanin-24 (Tspan-24). Like other tetraspanin family members, CD151 contains four hydrophobic transmembrane domains, two short cytoplasmic tails, and one small and one large extracellular loop. CD151 is expressed by many different cell types such as platelets, immature hematopoietic cells, megakaryocytes, keratinocytes, epithelial cells, muscle cells, Schwann cells, vascular endothelium, as well as epithelial cells. It complexes with integrins and other transmembrane 4 superfamily proteins and is involved in cellular processes including cell adhesion and regulates integrin trafficking and function. CD151 enhances cell motility, invasion, and metastasis of tumor cells.
Additional information: Clone REA265 displays negligible binding to Fc receptors.

Alternative names

GP27, MER2, PETA-3, RAPH, SFA1, TSPAN24

Detailed product information

Technical specifications

CloneREA265
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody (S), human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Specieshuman
AntigenCD151
Alternative names of antigenGP27, MER2, PETA-3, RAPH, SFA1, TSPAN24
Molecular mass of antigen [kDa]28
Distribution of antigenepithelial cells, platelets, keratinocytes, Schwann cells
Entrez Gene ID977
RRIDAB_2655231, AB_2655232, AB_2655233, AB_2655234, AB_2655235, AB_2655236, AB_2655237, AB_2655230

Resources for CD151 Antibody, anti-human, REAfinity™

Certificates

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to search for Certificates of Analysis (CoA) by lot number.

References for CD151 Antibody, anti-human, REAfinity™

Publications

  1. Karamatic, C. V. et al. (2004) CD151, the first member of the tetraspanin (TM4) superfamily detected on erythrocytes, is essential for the correct assembly of human basement membranes in kidney and skin. Blood 104(8): 2217-2223
  2. Kohno, M. et al. (2002) CD151 enhances cell motility and metastasis of cancer cells in the presence of focal adhesion kinase. Int. J. Cancer 97(3): 336-343
  3. Chernousov, M. A. et al. (2013) Tetraspanins are involved in Schwann cell-axon interaction. J. Neurosci. Res. 91(11): 1419-1428

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