CD14 Antibody, anti-mouse,
REAlease
^®

Name: CD14 Antibody, anti-mouse, REAlease®
Availability: InStock

CD14 Antibody, anti-mouse,
REAlease
^®
Extended ValidationRecombinant

Clone:

REAL466

Type of antibody:

Releasable antibodies, Primary antibodies, Recombinant antibodies

Applications:

FC

Alternative names:

LPS receptor

Flow cytometry applications forCD14 Antibody, anti-mouse,
REAlease
^®

APC
FITC
PE

Conjugate:

APC

Recommended dilution:

1:50

Tested:

QC tested

Products used:

130-122-242

Protocols:

Cell surface staining protocol using REAlease™ antibodies

Description:

J774A.1 cells were stained with REAlease CD14 Complexes or with the corresponding REAL Control (left peak) and analyzed by flow cytometry using the MACSQuant^®Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide (PI) fluorescence.

Extended validation for CD14 Antibody, anti-mouse,
REAlease
^®

Sensitivity

Performance comparison

Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.

Flow cytometric comparison of different clones for CD14. J774A.1 cells were stained with CD14 antibodies and plotted against the side scatter. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD14. J774A.1 cells were stained with CD14 antibodies and plotted against the side scatter. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD14. J774A.1 cells were stained with CD14 antibodies and plotted against the side scatter. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD14. J774A.1 cells were stained with CD14 antibodies and plotted against the side scatter. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD14. J774A.1 cells were stained with CD14 antibodies and plotted against the side scatter. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD14. J774A.1 cells were stained with CD14 antibodies and plotted against the side scatter. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Flow cytometric comparison of different clones for CD14. J774A.1 cells were stained with CD14 antibodies and plotted against the side scatter. As a control, CD14 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant^® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.

Fixation data

To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.

No fixation

Post fixation

Comparison of staining pattern on non-fixed and fixed cells using CD14 (REAL466). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Comparison of staining pattern on non-fixed and fixed cells using CD14 (REAL466). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD14 Antibody, anti-mouse,
REAlease
^®

Overview

Clone REAL466 is an antibody fragment derived from the full CD14 antibody molecule. It displays no binding to Fc receptors. The recombinantly engineered antibody fragments are multimerized to form the REAlease Complex to bind markers with high avidity.

Clone REAL466 recognizes the mouse CD14 antigen, a cell surface glycoprotein preferentially expressed on monocytes/macrophages, but also on granulocytes, dendritic cells, Kupffer cells, and hepatocytes. In association with toll-like receptors TLR 4 and MD-2, CD14 is a highly specific co-receptor for bacterial lipopolysaccharide (LPS) and for a variety of other pathogen-associated molecular patterns of microbial sources. The association of CD14 with LPS is mediated by the LPS-binding protein (LBP).

The REAlease Kits consist of the respective fluorochrome-conjugated REAlease Complexes and the REAlease Support Kit for removal of the REAlease Complexes and optional relabeling with different fluorochrome-conjugated REAlease Complexes.

Alternative names

LPS receptor

Detailed product information

Technical specifications

Clone	REAL466
Clonality	monoclonal
Isotype control	Control Antibody
Host	cell line
Type of antibody	Releasable antibodies, Primary antibodies, Recombinant antibodies
Species	mouse
Antigen	CD14
Alternative names of antigen	LPS receptor
Distribution of antigen	monocytes, macrophages, dendritic cells, granulocytes
RRID	AB_2801853, AB_2801856, AB_2811702, AB_2811703, AB_2801850

Resources for CD14 Antibody, anti-mouse,
REAlease
^®

Certificates

Please follow this

link

to search for Certificates of Analysis (CoA) by lot number.

Applications

Extended validation