Clone:
REA114
Type of antibody:
Primary antibodies, Recombinant antibodies
Isotype:
recombinant human IgG1
Applications:
FC
Alternative names:
IL3RA, CDw123, SUT-1

Extended validation for CD123 Antibody, anti-mouse, REAfinity™

Specificity

Epitope competition
In order to compare the epitope specificity of an antibody, the clone being used is compared with other known clones recognizing the same antigen in a competition assay.
Other clonesOverlap in epitope recognition with REA114
5B11++
Cells were incubated with an excess of purified unconjugated CD123 (REA114) antibody followed by staining with fluorochrome-conjugated antibodies of other known clones against the same marker. Based on the fluorescence signal obtained, the clones were identified as recognizing completely overlapping (++), partially overlapping (+), or completely different epitopes (-) of the marker.

Sensitivity

Performance comparison
Selected fluorochrome conjugated antibodies from Miltenyi Biotec were compared to commercially available hybridoma clones in flow cytometry analysis.
View details
Flow cytometric comparison of different clones for CD123. C57BL/6 Mouse bone marrow were stained with CD123 antibodies and with a suitable counterstaining. As a control, CD123 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD123. C57BL/6 Mouse bone marrow were stained with CD123 antibodies and with a suitable counterstaining. As a control, CD123 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD123. C57BL/6 Mouse bone marrow were stained with CD123 antibodies and with a suitable counterstaining. As a control, CD123 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD123. C57BL/6 Mouse bone marrow were stained with CD123 antibodies and with a suitable counterstaining. As a control, CD123 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD123. C57BL/6 Mouse bone marrow were stained with CD123 antibodies and with a suitable counterstaining. As a control, CD123 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
View details
Flow cytometric comparison of different clones for CD123. C57BL/6 Mouse bone marrow were stained with CD123 antibodies and with a suitable counterstaining. As a control, CD123 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Flow cytometric comparison of different clones for CD123. C57BL/6 Mouse bone marrow were stained with CD123 antibodies and with a suitable counterstaining. As a control, CD123 antibody staining was omitted and cells were measured in the same channels. Flow cytometry was performed with the MACSQuant® Analyzer. Cell debris, dead cells, and cell doublets were excluded from the analysis based on scatter signals and 4',6-diamidino-2-phenylindole (DAPI) fluorescence. No FcR Blocking Reagent was used. The recommended titers of respective antibodies from different suppliers were used.
Fixation data
To provide an indication on how an antibody performs after fixation of cells, in-house data on staining results before and after fixation with 3.7% formaldehyde using Miltenyi Biotec antibodies are provided. Different experimental settings may lead to different results.
No fixation
Post fixation
View details
Comparison of staining pattern on non-fixed and fixed cells using CD123 (REA114). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD123 (REA114). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD123 (REA114). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
View details
Comparison of staining pattern on non-fixed and fixed cells using CD123 (REA114). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.
Comparison of staining pattern on non-fixed and fixed cells using CD123 (REA114). The performance of the antibody after fixation was tested by comparing the staining pattern on fresh (no fixation) versus fixed (post fixation) cells.

Specifications for CD123 Antibody, anti-mouse, REAfinity™

Overview

Clone REA114 recognizes CD123, the alpha chain of the IL-3 receptor. CD123 is 60–70 kDa membrane glycoprotein and interacts in mice with common beta chain (betac) and beta IL-3 subunits to form functional heterodimeric IL-3 receptors. CD123 alone can bind IL-3 with low affinity, however a high affinity binding is displayed by the heterodimeric forms. Besides ligand binding, the alpha chain of IL-3R was also shown to be necessary for the activation of STAT-5. Expression of CD123 is widely found in hematopoietic cells and in addition on basophils, mast cells, and megakaryocytes. AKR, RF, A/J, and NZB mouse strains carry a 5-bp deletion in the IL-3Ra gene and thus lack the expression of CD123.
Additional information: Clone REA114 displays negligible binding to Fc receptors.

Alternative names

IL3RA, CDw123, SUT-1

Detailed product information

Technical specifications

CloneREA114
Clonalitymonoclonal
Isotyperecombinant human IgG1
Isotype controlREA Control Antibody, human IgG1
Hosthuman cell line
Type of antibodyPrimary antibodies, Recombinant antibodies
Speciesmouse
AntigenCD123
Alternative names of antigenIL3RA, CDw123, SUT-1
Molecular mass of antigen [kDa]42
Distribution of antigenB cells, granulocytes, monocytes, macrophages, endothelial cells, mast cells, megakaryocytes, red blood cells, eosinophils, basophils, bone marrow, brain, placenta
Entrez Gene ID16188
RRIDAB_2654775, AB_2654776, AB_2654777, AB_2654778, AB_2654779, AB_2654780, AB_2654781, AB_2654774

Resources for CD123 Antibody, anti-mouse, REAfinity™

Certificates

Please follow this
link
to search for Certificates of Analysis (CoA) by lot number.

References for CD123 Antibody, anti-mouse, REAfinity™

Publications

  1. Hara, T. et al. (1995) Interleukin-3 (IL-3) poor-responsive inbred mouse strains carry the identical deletion of a branch point in the IL-3 receptor alpha subunit gene. Blood 85: 2331-2336
  2. Reddy, E. P. et al. (2000) IL-3 signaling and the role of Src kinases, JAKs and STATs: a covert liaison unveiled. Oncogene 19(21): 2532-2547
  3. Lehmann, C. et al. (2014) Longitudinal analysis of distribution and function of plasmacytoid dendritic cells in peripheral blood and gut mucosa of HIV infected patients. J. Infect. Dis. 209: 940-949

Related products for
CD123 Antibody, anti-mouse, REAfinity™

2 products available

Seems like you are coming from USA!
Do you want to visit our website in your country?