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Immunofluorescence analysis of c-myc-BDCA-2 expressing CHO cells. CHO cells transiently expressing c-myc-BDCA-2 were analyzed by fluorescence microscopy 24 hours after transfection. A) Double staining of c-myc-BDCA-2 with Anti-c-myc-FITC and Anti-BDCA-2-Biotin/Anti-Biotin-Alexa 546. B) Double staining of c-myc-BDCA-2 with Anti-c-myc-Biotin/Anti-Biotin-Alexa 546 and BDCA-2-FITC. Red: Alexa 546-labeling, green: FITC-labeling, yellow: FITC-Alexa 546 overlay.
Immunofluorescence analysis of c-myc-BDCA-2 expressing CHO cells. CHO cells transiently expressing c-myc-BDCA-2 were analyzed by fluorescence microscopy 24 hours after transfection. A) Double staining of c-myc-BDCA-2 with Anti-c-myc-FITC and Anti-BDCA-2-Biotin/Anti-Biotin-Alexa 546. B) Double staining of c-myc-BDCA-2 with Anti-c-myc-Biotin/Anti-Biotin-Alexa 546 and BDCA-2-FITC. Red: Alexa 546-labeling, green: FITC-labeling, yellow: FITC-Alexa 546 overlay.
HEK 293 cells transiently expressing c-myc-BDCA-2 were lysed and c-myc-BDCA-2 was purified using the μMACS Anti-c-myc Isolation Kit. 1/100, 1/500, and 1/1000 of the eluate were subjected to SDS-polyacrylamide gel electrophoresis, blotted onto a PVDF membrane and detected using Anti-c-myc-HRP antibody (1:10,000, 1 h, room temperature) and ECL reagent.
Clone | SH1-26E7.1.3 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG1 |
Isotype control | Isotype Control Antibody, mouse IgG1 |
Host | mouse |
Type of antibody | Primary antibodies, Epitope tag antibodies |
Species | any |
Antigen | c-myc |
Molecular mass of antigen [kDa] | 1.2 |
Distribution of antigen | any cell type |
RRID | AB_2751321, AB_2889650, AB_2889649, AB_871937, AB_2751329 |
Clone | SH1-26E7.1.3 |
---|---|
Clonality | monoclonal |
Isotype | mouse IgG1 |
Isotype control | Isotype Control Antibody, mouse IgG1 |
Host | mouse |
Type of antibody | Primary antibodies, Epitope tag antibodies |
Species | any |
Antigen | c-myc |
Molecular mass of antigen [kDa] | 1.2 |
Distribution of antigen | any cell type |
RRID | AB_2751321, AB_2889650, AB_2889649, AB_871937, AB_2751329 |
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