Isolation of PTK7 (CCK-4) + cells from PBMCs using the Anti-PTK7 (CCK-4) MicroBead Kit and a MiniMACS™ Separator with MS Columns. Aliquots are fluorescently stained (A) before and (B) after magnetic separation with CD34-FITC, CD303 (BDCA-2)-PE and CD141 (BDCA-3)-APC for identification of hematopoietic stem cells (HPCs), plasmacytoid dendritic cells (PDCs), and type-2 myeloid dendritic cells (MDC2), respectively. Before and after separation, cells were gated on R1 (PDCs), R2 (HPCs), and R3 (MDC2s). Due to specific expression of CD303 (BDCA-2), CD141 (BDCA-3), and CD34 there is no overlap between the different PTK7-expressing cell types, and PDCs, HPCs, and MDC2s are clearly discriminated. |
A) Before magnetic separation |
Identification of PDCs and HPCs | Identification of MDC2s |
  View details Figure 1 Isolation of PTK7 (CCK-4) + cells from PBMCs using the Anti-PTK7 (CCK-4) MicroBead Kit and a MiniMACS™ Separator with MS Columns. Aliquots are fluorescently stained (A) before and (B) after magnetic separation with CD34-FITC, CD303 (BDCA-2)-PE and CD141 (BDCA-3)-APC for identification of hematopoietic stem cells (HPCs), plasmacytoid dendritic cells (PDCs), and type-2 myeloid dendritic cells (MDC2), respectively. Before and after separation, cells were gated on R1 (PDCs), R2 (HPCs), and R3 (MDC2s). Due to specific expression of CD303 (BDCA-2), CD141 (BDCA-3), and CD34 there is no overlap between the different PTK7-expressing cell types, and PDCs, HPCs, and MDC2s are clearly discriminated. |  View details Figure 1 Isolation of PTK7 (CCK-4) + cells from PBMCs using the Anti-PTK7 (CCK-4) MicroBead Kit and a MiniMACS™ Separator with MS Columns. Aliquots are fluorescently stained (A) before and (B) after magnetic separation with CD34-FITC, CD303 (BDCA-2)-PE and CD141 (BDCA-3)-APC for identification of hematopoietic stem cells (HPCs), plasmacytoid dendritic cells (PDCs), and type-2 myeloid dendritic cells (MDC2), respectively. Before and after separation, cells were gated on R1 (PDCs), R2 (HPCs), and R3 (MDC2s). Due to specific expression of CD303 (BDCA-2), CD141 (BDCA-3), and CD34 there is no overlap between the different PTK7-expressing cell types, and PDCs, HPCs, and MDC2s are clearly discriminated. |
Discrimination between PTK7-expressing cell types |
 View details Figure 1 Isolation of PTK7 (CCK-4) + cells from PBMCs using the Anti-PTK7 (CCK-4) MicroBead Kit and a MiniMACS™ Separator with MS Columns. Aliquots are fluorescently stained (A) before and (B) after magnetic separation with CD34-FITC, CD303 (BDCA-2)-PE and CD141 (BDCA-3)-APC for identification of hematopoietic stem cells (HPCs), plasmacytoid dendritic cells (PDCs), and type-2 myeloid dendritic cells (MDC2), respectively. Before and after separation, cells were gated on R1 (PDCs), R2 (HPCs), and R3 (MDC2s). Due to specific expression of CD303 (BDCA-2), CD141 (BDCA-3), and CD34 there is no overlap between the different PTK7-expressing cell types, and PDCs, HPCs, and MDC2s are clearly discriminated. |
B) Enriched PTK7 (CCK-4)+ cells |
Identification of PDCs and HPCs | Identification of MDC2s |
 View details Figure 1 Isolation of PTK7 (CCK-4) + cells from PBMCs using the Anti-PTK7 (CCK-4) MicroBead Kit and a MiniMACS™ Separator with MS Columns. Aliquots are fluorescently stained (A) before and (B) after magnetic separation with CD34-FITC, CD303 (BDCA-2)-PE and CD141 (BDCA-3)-APC for identification of hematopoietic stem cells (HPCs), plasmacytoid dendritic cells (PDCs), and type-2 myeloid dendritic cells (MDC2), respectively. Before and after separation, cells were gated on R1 (PDCs), R2 (HPCs), and R3 (MDC2s). Due to specific expression of CD303 (BDCA-2), CD141 (BDCA-3), and CD34 there is no overlap between the different PTK7-expressing cell types, and PDCs, HPCs, and MDC2s are clearly discriminated. |  View details Figure 1 Isolation of PTK7 (CCK-4) + cells from PBMCs using the Anti-PTK7 (CCK-4) MicroBead Kit and a MiniMACS™ Separator with MS Columns. Aliquots are fluorescently stained (A) before and (B) after magnetic separation with CD34-FITC, CD303 (BDCA-2)-PE and CD141 (BDCA-3)-APC for identification of hematopoietic stem cells (HPCs), plasmacytoid dendritic cells (PDCs), and type-2 myeloid dendritic cells (MDC2), respectively. Before and after separation, cells were gated on R1 (PDCs), R2 (HPCs), and R3 (MDC2s). Due to specific expression of CD303 (BDCA-2), CD141 (BDCA-3), and CD34 there is no overlap between the different PTK7-expressing cell types, and PDCs, HPCs, and MDC2s are clearly discriminated. |
Discrimination between PTK7-expressing cell types |
 View details Figure 1 Isolation of PTK7 (CCK-4) + cells from PBMCs using the Anti-PTK7 (CCK-4) MicroBead Kit and a MiniMACS™ Separator with MS Columns. Aliquots are fluorescently stained (A) before and (B) after magnetic separation with CD34-FITC, CD303 (BDCA-2)-PE and CD141 (BDCA-3)-APC for identification of hematopoietic stem cells (HPCs), plasmacytoid dendritic cells (PDCs), and type-2 myeloid dendritic cells (MDC2), respectively. Before and after separation, cells were gated on R1 (PDCs), R2 (HPCs), and R3 (MDC2s). Due to specific expression of CD303 (BDCA-2), CD141 (BDCA-3), and CD34 there is no overlap between the different PTK7-expressing cell types, and PDCs, HPCs, and MDC2s are clearly discriminated. |
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