The Anti-Integrin α-7 MicroBeads, mouse have been developed for the direct isolation of Integrin α-7 positive cells from various sources such as skeletal muscle.

Data and images for Anti-Integrin α-7 MicroBeads, mouse

Figures

Figure 1

Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit (# 130-098-305) in combination with the gentleMACS Dissociator. Non-satellite cells were depleted using the Satellite Cell Isolation Kit (# 130-104-268) followed by enrichment of satellite cells using Anti-Integrin α-7 MicroBeads. Cells were fluorescently stained with lineage markers (CD31/CD45/CD11b/Sca-1) in the FITC channel, Anti-Integrin α-7-PE, and CD34-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit (# 130-098-305) in combination with the gentleMACS Dissociator. Non-satellite cells were depleted using the Satellite Cell Isolation Kit (# 130-104-268) followed by enrichment of satellite cells using Anti-Integrin α-7 MicroBeads. Cells were fluorescently stained with lineage markers (CD31/CD45/CD11b/Sca-1) in the FITC channel, Anti-Integrin α-7-PE, and CD34-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
View details

Figure 1

Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit (# 130-098-305) in combination with the gentleMACS Dissociator. Non-satellite cells were depleted using the Satellite Cell Isolation Kit (# 130-104-268) followed by enrichment of satellite cells using Anti-Integrin α-7 MicroBeads. Cells were fluorescently stained with lineage markers (CD31/CD45/CD11b/Sca-1) in the FITC channel, Anti-Integrin α-7-PE, and CD34-APC and analyzed by flow cytometry using the MACSQuant
®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-Integrin α-7 MicroBeads, mouse

Overview

The Anti-Integrin α-7 MicroBeads, mouse have been developed for the direct isolation of Integrin α-7 positive cells from various sources such as skeletal muscle.

Detailed product information

Background information

Integrin alpha 7 (ITGA7) belongs to the integrin family of adhesion molecules. Integrins participate in important cell-cell and cell-extracellular matrix interactions in a diverse range of biological processes. ITGA7 is a heterodimer consisting of an α and a β subunit and is mainly expressed in skeletal and cardiac muscle. In skeletal muscle, ITGA7 is a marker for satellite cells. It is a specific cellular receptor for the basement membrane protein laminin-1, as well as for the laminin isoforms-2 and -4 and is involved in differentiation and migration processes during myogenesis. Absence of ITGA7 results in muscular dystrophy. It is also required to promote contractile phenotype acquisition in differentiated airway smooth muscle cells.

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