Anti-Fibroblast MicroBeads, human

Name: Anti-Fibroblast MicroBeads, human
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Anti-Fibroblast MicroBeads, human

Anti-Fibroblast MicroBeads have been developed for the positive selection or depletion of human fibroblasts.

Product data and images for Anti-Fibroblast MicroBeads, human

Figures

Figure 1

Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator.

Unseparated fraction	Non-fibroblast cells in flow-through
View details Anti-Fibroblast MicroBeads, human Figure 1 Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator.	View details Anti-Fibroblast MicroBeads, human Figure 1 Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator.
Isolated fibroblasts
View details Anti-Fibroblast MicroBeads, human Figure 1 Positive selection of fibroblasts from a mixture of cells of an epithelial tumor cell line and normal human dermal fibroblasts using Anti-Fibroblast MicroBeads, an MS Column, and a MiniMACS™ Separator.

Specifications for Anti-Fibroblast MicroBeads, human

Overview

Anti-Fibroblast MicroBeads have been developed for the positive selection or depletion of human fibroblasts.

Detailed product information

Background information

Anti-Fibroblast MicroBeads are based on a fibroblast-specific antigen.

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This antigen is also known to be expressed on mesenchymal stem cells, also known as mesenchymal stromal cells, (MSCs) in the bone marrow.

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Applications

Depletion of human fibroblasts that are used as feeder cells to support the growth of ES or iPS cells
Depletion of fibroblasts after reprogramming⁵
Isolation of MSCs from bone marrow aspirate
Removal of fibroblastic cells from bone marrow cultures
Positive selection of fibroblasts from cell mixtures

Columns

For positive selection: MS, LS, XS, or autoMACS

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Columns. For depletion: LD, D, or autoMACS Columns.

Resources for Anti-Fibroblast MicroBeads, human

Documents and Protocols

App notes/customer reports

The expression of novel mesenchymal/stromal lineage transcripts in uncultured CD271-positive MSCs: differences from culture-expanded MSCs.

References for Anti-Fibroblast MicroBeads, human

Publications

Fearns, C. and Dowdle, E. B. (1992)
The desmoplastic response: induction of collagen synthesis by melanoma cells
in vitro
.
Int. J. Cancer 50: 621-627
Jones, E. A. et al. (2002) Isolation and characterization of bone marrow multipotential mesenchymal progenitor cells. Arthritis Rheum 46: 3349-3360
Jones, E. A. et al. (2006) Optimization of a flow cytometry-based protocol for detection and phenotypic characterization of multipotent mesenchymal stromal cells from human bone marrow. Cytometry B Clin. Cytom. 70: 391-399
Jones et al. (2007) MACS&more 11(1): 22-25
Paul, D. et al. (2015) Automated, high-throughput derivation, characterization and differentiation of induced pluripotent stem cells. Nat. Methods 12(9): 885-892

Data and images