Anti-ACSA-2 MicroBead Kit, mouse

Name: Anti-ACSA-2 MicroBead Kit, mouse
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Anti-ACSA-2 MicroBead Kit, mouse

Anti-ACSA-2 MicroBeads (ACSA-2: astrocyte cell surface antigen-2) have been developed for the isolation or depletion of primary mouse astrocytes based on the expression of the ACSA-2 antigen. The protocol can be performed within only one hour.

Product data and images for Anti-ACSA-2 MicroBead Kit, mouse

Figures

Figure 1

ACSA-2

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cells were isolated from P4 CD-1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, Anti-ACSA-2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorecently stained with Anti-ACSA-2-PE and analyzed by flow cytometry using the MACSQuant

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Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Before separation	ACSA-2 ⁺ cells
View details Anti-ACSA-2 MicroBead Kit, mouse Figure 1 ACSA-2 ⁺ cells were isolated from P4 CD-1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, Anti-ACSA-2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorecently stained with Anti-ACSA-2-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.	View details Anti-ACSA-2 MicroBead Kit, mouse Figure 1 ACSA-2 ⁺ cells were isolated from P4 CD-1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, Anti-ACSA-2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorecently stained with Anti-ACSA-2-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
ACSA-2 ^– cells
View details Anti-ACSA-2 MicroBead Kit, mouse Figure 1 ACSA-2 ⁺ cells were isolated from P4 CD-1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, Anti-ACSA-2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorecently stained with Anti-ACSA-2-PE and analyzed by flow cytometry using the MACSQuant ^® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.

Specifications for Anti-ACSA-2 MicroBead Kit, mouse

Overview

Anti-ACSA-2 MicroBeads (ACSA-2: astrocyte cell surface antigen-2) have been developed for the isolation or depletion of primary mouse astrocytes based on the expression of the ACSA-2 antigen. The protocol can be performed within only one hour.

Detailed product information

Background information

The ACSA-2 antigen is specifically expressed on GLAST (ACSA-1) positive astrocytes and is therefore an exclusive marker of astrocytes in the developing and neonatal mouse central nervous system (CNS). The Anti-ACSA-2 MicroBeads allow also for purification of astrocytes that show weak GLAST (ACSA-1) expression.

The percentage of ACSA-2 positive astrocytes differs according to the mouse age and the brain region used for the cell isolation.

The isolation of primary astrocytes was tested particularly on dissociated postnatal CD-1 mouse brain tissue derived from animals younger than postnatal day 8 (P8). The protocol can be performed in only one hour. The astrocytes show excellent morphology in culture using MACS® Neuro Medium, MACS® NeuroBrew-21 with P/S and L-glutamine.

Resources for Anti-ACSA-2 MicroBead Kit, mouse

Documents and Protocols

Brochures/posters

Neuroscience - Inspiring technologies for creative scientists

Scientific posters

Efficient isolation of viable primary neural cells from adult murine brain tissue based on a novel automated tissue dissociation protocol

Scientific posters

Highly efficient dissociation of distinct adult rodent brain regions ensures high cell viability and recovery for successful downstream processing

Scientific posters

A novel dissociation procedure allows efficient immunomagnetic isolation of astrocytes, oligodendrocytes, and neurons from adult rodent brain tissue

Scientific posters

Characterization of the cytokine secretion profile of highly purified, functional astrocytes

Protocols

Isolation of astrocytes from adult mouse brain

References for Anti-ACSA-2 MicroBead Kit, mouse

Publications

Feldmann, M. et al. (2014) Isolating astrocytes and neurons sequentially from postnatal murine brains with a magnetic cell separation technique. J. Biol. Methods 1(2): e11
Sharma, K. et al. (2015) Cell type- and brain region-resolved mouse brain proteome. Nat. Neurosci. 18(12): 1819-1831
Holt, L. M. and Olsen, M. L. (2016) Novel applications of magnetic cell sorting to analyze cell-type specific gene and protein expression in the central nervous system. PLoS One 11(2): e0150290
G. Kantzer, C. et al. (2017) Anti-ACSA-2 defines a novel monoclonal antibody for prospective isolation of living neonatal and adult astrocytes. Glia 65(6): 990-1004
Batiuk, M. Y. et al. (2017) An immunoaffinity-based method for isolating ultrapure adult astrocytes based on ATP1B2 targeting by the ACSA-2 antibody. J. Biol. Chem. 292(21): 8874-8891

Data and images