• PBE buffer: Prepare a solution containing phosphate-buffered saline (PBS), pH 7.2, 0.5% bovine serum albumin (BSA), and 2 mM EDTA by diluting MACS® BSA Stock Solution (# 130‑091‑376) 1:20 with autoMACS® Rinsing Solution (# 130‑091‑222). Keep buffer cold (2−8 °C). Always use freshly prepared buffer. Do not use autoMACS Running Buffer or MACSQuant® Running Buffer as they contain a small amount of sodium azide that could affect the results.
  ▲ Note: EDTA can be replaced by other supplements such as anticoagulant citrate dextrose formula-A (ACD-A) or citrate phosphate dextrose (CPD). BSA can be replaced by other proteins such as mouse or rat serum albumin, mouse or rat serum, or FBS. Buffers or media containing Ca²⁺ or Mg²⁺ are not recommended for use.
  

• Skeletal Muscle Dissociation Kit, mouse and rat (# 130-098-305)
• Cell culture medium without fetal bovine serum (FBS), e.g., DMEM
• gentleMACS™ Dissociator (# 130-093-235), gentleMACS OctoDissociator (# 130-095-937), or gentleMACS Octo Dissociator with Heaters (# 130-096-427)
• gentleMACS C Tubes (# 130-093-237, # 130-096-334)
• MACSmix™ Tube Rotator (# 130-090-753) in combination with an incubator at 37 °C
  
• MACS SmartStrainers (70 μm) (# 130-098-462)
  
• (Optional) Red Blood Cell Lysis Solution (10×) (# 130-094-183) 

• (Optional) ART. 1000 REACH™ pipet tips (Molecular BioProducts, Inc.) for removal of dissociated material from the closed C Tubes

• Satellite Cell Isolation Kit, mouse (# 130-104-268)
• PBS buffer
• Pre-Separation Filters, 70 μm (# 130-095-823) to remove cell clumps
  
• (Optional) Basic FGF/FGF2, e.g., Human FGF-2, premium grade (# 130-093-840) for culture of satellite cells
• (Optional) Inside Stain Kit (# 130-090-477) to detect intracellular markers, such as Pax7
• (Optional) Propidium Iodide Solution (# 130-093-233) or 7-AAD for flow cytometric exclusion of dead cells without fixation
• (Optional) Anti-Integrin α-7 MicroBeads, mouse (# 130-104-261) to further increase the purity of isolated satellite cells, e.g., for direct molecular analysis, such as mRNA expression profiling.
• MACS Columns and MACS Separators: For optimal purity and recovery use an LS Column. Depletion can also be performed using the autoMACS Pro Separator.

▲ Note: Column adapters are required to insert certain columns into the VarioMACS™ or SuperMACS™ II Separators. For details refer to the respective MACS Separator data sheet.