Application protocol

Multicolor flow cytometric analysis of granulocytes from mouse spleen

This application protocol describes the flow cytometric analysis of granulocytes after spleen dissociation from healthy C57BL/6 mice. Viable single cells from mouse spleens are easily obtained using the gentleMACS™ Technology. For downstream flow cytometric analysis of different granulocyte populations such as neutrophils, basophils, and eosinophils, we have designed two validated multicolor flow cytometry panels, using our REAfinity™ Recombinant Antibodies and Viobility™ Fixable Dyes. In addition, we provide optimized gating strategies for the analysis of granuloycte populations from mouse spleen.

Materials

The following is a listing of reagents, instruments, and consumables needed for each step of this protocol. These products are for research use only. 
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Protocol


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This protocol includes detailed instructions for preparing necessary reagents and materials and then carrying out each step.