Transduction of human cell lines with purified adenoviral vector.
HuT78 and A549 cells were magnetically labeled with CD30 and CD44 MicroBeads, respectively. In parallel, purified adenoviral vector particles encoding GFP were complexed with MACSductin Reagent.
The A549 cells were then transduced in MS Columns at a pMOI (physical particles per cell) of 50 while HuT78 cells were transduced at a pMOI of 500. GFP expression of transduced cells was increased up to 30-fold 24 hours post-transduction as measured by flow cytometry (data courtesy of Dr. Florian Kreppel and Dr. Sigrid Espenlaub, Department of Gene Therapy, University of Ulm, Germany).