With ultra high content imaging comes an ultra high amount of data to handle.
Qi Tissue Image Analysis Software was specifically developed to analyze the enormous data stacks obtained by the MACSima™ Imaging System. Its versatility and intuitive user interface make it the perfect tool to analyze the multidimensional data sets gathered during MICS (MACSima imaging cyclic staining) experiments, and to extract the underlying information in an easy and comprehensive manner.
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Qi Tissue - in detail
Simply load your data into the Qi Tissue Image Analysis Software and immediately get a clear overview of your ultra high content experiment data set. With Qi Tissue you have all your analysis tools right at your fingertips and you can customize the display to fit your needs. Navigating through these vast amounts of data quickly becomes effortless and instinctive.
Data visualization is the first step in analyzing your data. With the Qi Tissue Image Analysis Software, you can easily navigate through all your markers and create different composites by simply activating and deactivating markers. Move your cursor over the image to get a first look at the intensity levels of your displayed markers in real-time.
The extraordinarily high number of markers obtained in a MICS experiment facilitates the accurate recognition of nuclei and cell boundaries, making segmentation faster and more reliable. In this way, correlation of the fluorescence values within your image to certain cells becomes straightforward, allowing the accurate comparison of expression patterns, identification and definition of cell populations, calculation of clusters, and more.
Gating is a well-known and extremely useful tool regularly employed by flow cytometry researchers. Qi Tissue Image Analysis Software extends the function of this tool by allowing you to gate and sub-gate cells of interest based on a scatter plot, but also by selecting cells in a certain part of the tissue in the image viewer. Select your cells of interest in the plot and see them immediately highlighted on your tissue, or select the tissue section of interest to have it highlighted in your plot.
Use the high amount of data obtained by the MACSima Imaging System to perform clustering analysis with the option of visualizing clusters by location directly on your tissue, or on your scatter plots. Show your expression profiles in heatmaps or reduce the dimensionality of your data by calculating t-SNE plots for better data exploration and visualization. All data and visualizations can easily be extracted and used for any kind of follow-up analysis or directly in high-end publications.
Packed with many more innovative and practical features, Qi Tissue Image Analysis Software is a powerful tool that puts the entire potential of high content MICS experiments at your fingertips, effortlessly.
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