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PBMCs were stimulated with 1 µg/mL SEB for 16 hours. The responding cells were stained according to secretion of IL-4 using the IL-4 Secretion Assay - Detection Kit. In the stimulated sample, 0.307% of all CD4+
T cells secreted IL-4. In the unstimulated control sample, 0.004% of all CD4+
T cells secreted IL-4.
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