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Human peripheral blood mononuclear cells (PBMCs) were stained with CD39 antibodies or with the corresponding isotype control antibodies (left images) as well as with CD4 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Furthermore, to evaluate CD39 expression on regulatory T cells, CD4+
T cells were enriched using the CD4+
T Cell Isolation Kit, human. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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