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Mouse splenocytes were stained with CD28 antibodies conjugated to PE (A) and Biotin (B) as well as with CD3ε-FITC and analyzed by flow cytometry. Cells stained with CD28-Biotin were stained with Anti-Biotin-PE in addition. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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