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CD266 transfected mouse lymphoma L5178Y cells were stained with CD266 (FN14) antibodies or with the corresponding isotype control antibodies (left peak) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem-conjugates.
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