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Splenocytes from BALB/c mice, either left unstimulated (left images) or stimulated with 1 µg/mL Anti-IgM antibodies and 5 µg/mL CD40 antibodies for 3 days, were stained with CD252 (OX40L) antibodies as well as with CD19 antibodies. Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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