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Liver cells of C57BL/6 mice were dissociated with the Liver Dissociation Kit, mouse. Cells were then stained with CD146 (LSEC) antibodies or with the corresponding REA Control antibodies (left images) as well as with CD49b antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescencer or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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