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Human peripheral blood cells after erythrocyte lysis were fixed and permeabilized using the Cell Signaling Buffer Set A followed by intracellular staining with Anti-PLC-γ2 antibodies or with the corresponding REA Control (I) antibodies (left peak). Flow cytometry was performed with the MACSQuant®
cells were pregated for the analysis. Cell debris were excluded from the analysis based on scatter signals.
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