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Splenocytes from C57BL/6 mice were stained with Anti-Ly-49H antibodies as well as with CD49b antibodies and analyzed by flow cytometry using the MACSQuant®
cells were excluded from the analysis. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence or 4',6-diamidino-2-phenylindole (DAPI) fluorescence, as in the case of tandem conjugates.
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