Find the products and resources you are looking for!
Miltenyi Biotec distribution:
Mouse spleen cells were incubated with or without PMA/Ionomycin for 6 hours. After 2 hours, brefeldin A was added. The cells were harvested, fixed, permeabilized, and intracellularly stained with Anti-IL-5-PE, CD4-FITC, and CD154-APC. Cells were analyzed by flow cytometry using the MACSQuant® Analyzer. Gating was performed according to the CD4 expression and side scatter properties of the cells.
Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.