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Human peripheral blood mononuclear cells (PBMCs) were stimulated with 1 µg/mL LPS and 1 µg/mL Brefeldin at 37 °C for 5 hours. Cells were then fixed, permeabilized, and stained with Anti-IL-1α antibodies or with the corresponding isotype control (left image) as well as with CD14 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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