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T cells were either left unstimulated (left images) or stimulated with CD3/CD28/Anti-IL-4 antibodies and IL-2/IL-12 for 6 days. Cells were then restimulated with 50 ng/mL phorbol 12-myristate 13-acetate (PMA) and 1 µg/mL ionomycin for 1 hour and stained with Anti-IL-12R β2 antibodies as well as with CD3 antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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