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Human JEG-3 cells were fixed, permeabilized and stained with Anti-HLA-G antibodies or with the corresponding isotype control antibodies (left peak). The FcR Blocking Reagent has been used to avoid Fc receptor-mediated antibody labeling. The Tandem Signal Enhancer has been used to increase binding specificity of tandem-dye–conjugated antibodies. Flow cytometry was performed with the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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