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Mouse 1881 pre–B lymphoma cells were treated with demecolcine for 4 hours, fixed, and permeabilized using the Cell Signaling Buffer Set A. Cells were then stained with Anti-Histone H3 pS28 antibodies or with the corresponding REA Control (I) antibodies (left images). After the staining, the cells were treated with 1 mg/mL RNase for 30 minutes and then stained with 4',6-diamidino-2-phenylindole (DAPI) or propidium iodide. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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