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Mouse brain tissue postnatal day 7 was dissociated using the Neural Tissue Dissociation Kit (T) and the gentleMACS™ Dissociator. Brain cells were stained using Anti-GLAST (ACSA‑1) antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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