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A431 cells, either left unstimulated (left peak) or stimulated with 200 ng/mL human EGF for 5 minutes at 37 °C, were fixed and permeabilized using the Cell Signaling Buffer Set A followed by an intracellular staining with Anti-EGF Receptor pY1173 antibodies. Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris was excluded from the analysis based on scatter signals.
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