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Human peripheral blood mononuclear cells (PBMCs), either left unstimulated (left images) or stimulated with LPS for 2 hours followed by an incubation with brefeldin A for 22 hours, were fixed, permeabilized, and stained with Anti-CCL7 (MCP-3) antibodies. Flow cytometry was performed using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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