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Human peripheral blood mononuclear cells (PBMCs), either left unstimulated (left image) or stimulated with purified anti-human IgM at 37 °C for 30 seconds, were fixed and permeabilized. Cells were then stained with Anti-BLNK pY84 antibodies as well as with CD20 antibodies and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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