VarioMACS™ Separator

VarioMACS™ Separator

The VarioMACS™ Separator gives the flexibility to separate small samples as well as samples of up to 2×10
9
total cells. Various MACS
®
Columns can be used with the height-adjustable VarioMACS Separator:
  • MS Columns for positive selection or depletion
  • LS Columns for positive selection or depletion
  • LD Columns for depletion
  • Whole Blood Columns for positive selection of cells directly from whole blood
The MS Column Adapter is required to insert MS Columns into the magnetic field of the VarioMACS Separator, and the LS Column Adapter is required to insert LS, LD, or Whole Blood Columns.

Background information

The VarioMACS™ Separator contains a powerful permanent magnet that induces a high-gradient magnetic field within MACS
®
Columns – a field strong enough to retain cells labeled with even small amounts of MACS MicroBeads.

Applications

In addition to many applications using MACS MicroBeads, the VarioMACS Separator, and the SuperMACS™ II Separator in combination with LD Columns
1
have been used for the enrichment of
Plasmodium falciparum–
infected erythrocytes without magnetic labeling
2
. The malaria parasites digest red blood cell hemoglobin, resulting in the formation of hemozoin. The paramagnetic properties of hemozoin allow magnetic isolation of red blood cells without the need for further labeling.
  • Selected references

    1. Uhlemann, A. C. et al. (2000) MACS&more 4(2): 7-8
    2. Staalsoe, T. et al. (1999)
      Detection of antibodies to variant antigens on
      Plasmodium falciparum
      -infected erythrocytes by flow cytometry.
      Cytometry 35(4): 329-336
Product options: 1
1
unit
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