Magnetic cell separation straight from blood or blood products

Immune cell isolation directly from blood with StraightFrom® MicroBeads

  • Isolation of pure immune cells directly from whole blood, buffy coat, LRSC, or Leukopak®
  • No need for density gradient centrifugation or erythrocyte lysis
  • Manual or automated isolation of multiple samples

StraightFrom® MicroBeads allow for the magnetic cell separation of various leukocyte subsets directly from  whole blood, buffy coat, leukocyte reduction system chamber (LRSC), or Leukopak® (StemExpress, LCC). The innovative StraightFrom Technology omits the time-consuming step of density gradient centrifugation. The isolated target cells are immediately ready for any cellular and molecular analysis.

The basic principle

Cell isolation with StraightFrom MicroBeads
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Isolation of target cells with StraightFrom MicroBeads is based on positive selection. Get your target cells in three easy steps: add the MicroBeads of choice, incubate the sample for magnetic labeling, and separate cells over a column. 

Leukocyte subsets can be purified either manually using a MACS® Separator or automated, using the MultiMACS™ Cell24 Separator Plus. Cells from whole blood can be isolated with the autoMACS® Pro Separator or the MultiMACS™ X.

The StraightFrom Protocol shortens the isolation of target cells to less than 30 minutes.
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Conventional protocols require the isolation of PBMCs by density gradient centrifugation before target cell isolation. The innovative StraightFrom Technology omits this time-consuming step delivering target cells in under 30 minutes.

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Video

How to isolate immune cells directly from blood

Follow this fast and easy protocol for magnetic isolation of immune cells directly from blood or blood products without the need for density gradient centrifugation.

Starting material

StraightFrom MicroBead Kits for the isolation of cells directly from whole blood, buffy coats, LRSCs, or Leukopak®.
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We offer tailored StraightFrom MicroBead Kits for cell isolation from whole blood, buffy coats, LRSCs, or Leukopak®. 

Depending on the starting material, volumes and leukocyte concentrations highly differ. All kits have been developed to meet the requirements in terms of cell purity and recovery rates.

Find the complete list of StraightFrom MicroBead Kits below.

Data

Isolation of CD4+ cells using StraightFrom MicroBeads

Magnetic isolation of CD4+ cells from a buffy coat sample using the StraightFrom Buffy Coat CD4 MicroBead Kit, human and the MultiMACS Cell24 Separator Plus.

Before separation
After separation

Resources

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Brochure

StraightFrom MicroBeads - Cell isolation directly from blood products directly from blood products

Download the brochure to get all the details on our StraightFrom MicroBead Technology.

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Scientific poster

Isolation of functional cells straight from buffy coat, LRSC, and Leukopak®

Maike Schlemminger, Silke Weber-Lohmann, Martin Kolnik*, Zhong Yu, Susanne Höher-Peters, and Gregor Winkels

Miltenyi Biotec, Bergisch Gladbach, Germany

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Webinar

Immune cell isolation without density gradient centrifugation

Learn from this webinar how to isolate immune cells straight from blood or blood products without density gradient centrifugation.

 

 

Selected reference

Rapid purification of billions of circulating CD19+ B cells directly from leukophoresis samples.

Ferrara, F. et al. (2018) N Biotechnol. 46:14-21 (PMID: 29870785)

 

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