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Enrichment of tumor cells from whole blood sample spiked with cells from a breast cancer cell line (SK-BR3) using StraightFrom®
Whole Blood CD326 (EpCAM) MicroBeads and the program “Posselwb” on the autoMACS®
Pro Separator. Cells are fluorescently stained with CD326 (EpCAM)-FITC and CD45-VioBlue®
and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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