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Mouse skeletal muscle was dissociated using the Skeletal Muscle Dissociation Kit (# 130-098-305) in combination with the gentleMACS Dissociator. Non-satellite cells were depleted using the Satellite Cell Isolation Kit (# 130-104-268) followed by enrichment of satellite cells using Anti-Integrin α-7 MicroBeads. Cells were fluorescently stained with lineage markers (CD31/CD45/CD11b/Sca-1) in the FITC channel, Anti-Integrin α-7-PE, and CD34-APC and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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