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As a global market leader with numerous subsidiaries and distributors, Miltenyi Biotec is committed to providing our customers around the world with the highest quality products. In addition to direct selling in more than 20 countries in North America, Europe and Asia/Pacific, Miltenyi Biotec also provides support for our customers through an extensive distributor network covering dozens of additional countries.
Single-cell suspensions from mouse or rat spleen were prepared using the gentleMACS™ Dissociator. CD90.1+
T cells were isolated from the single-cell suspensions using the CD90.1 MicroBeads, an autoMACS®
Column, and autoMACS Pro Separator. Cells were fluorescently stained with REAfinity™ Antibodies. Mouse cells were stained with CD45-FITC, CD45R(220)-PE-Vio®
770 and CD90.1-VioBlue®
(A, B) and rat cells were stained with CD90.1-VioBlue (C, D). Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.
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