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A single-cell suspension from mouse spleen was prepared using the gentleMACS™ Dissociator. CD8a+
T cells were isolated from this single-cell suspension using the CD8a (Ly-2) MicroBeads, an LS Column, and a QuadroMACS™ Separator. Cells were fluorescently stained with REAfinity™ Antibodies CD45-VioGreen™, CD4-VioBlue®
770 and analyzed by flow cytometry using the MACSQuant®
Analyzer. Viable leukocytes were gated for analysis based on scatter signals, 7-AAD Staining Solution fluorescence, and CD45 expression.
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