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cells were isolated, after re-expression of the AN2 antigen, from day 6 postnatal CD1 mouse brain tissue using the Neural Tissue Dissociation Kit (P), the gentleMACS™ Dissociator, FcR Blocking Reagent, mouse, the Anti-AN2 MicroBeads, a MiniMACS™ Separator, and an MS Column. Cells were fluorescently stained with Labeling Check Reagent-PE and analyzed by flow cytometry using the MACSQuant® Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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