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Monocytes were isolated from C57BL/6 bone marrow cells by using the Monocyte Isolation Kit (BM), mouse, an LS Column and a QuadroMACS Separator. The cells were fluorescently stained with CD11b-VioBlue, Lin cocktail (CD3ε-PE, CD45R (B220)-PE, Anti-NK1.1-PE, Anti-Ly-6G-PE, CD49b-PE), Anti-Ly-6C-FITC and Anti-Siglec-F-APC and analyzed by flow cytometry using the MACSQuant Analyser. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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