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cells were isolated from human PBMCs using the Diamond CD133 Isolation Kit, an LD Column, two MS Columns, a MidiMACS™ Separator, and a MiniMACS™ Separator. Cells were fluorescently stained with CD34-FITC, CD133/2 (293C3)-PE, CD45-APC, and Anti-Biotin-PE and analyzed using the MACSQuant® Analyzer.
PBMCs before separation
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