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cells were isolated from human cord blood MNCs by using the CD34 MultiSort MicroBeads, two LS Columns, and a MidiMACS™ Separator for the first separation. In the second separation step, the CD38 MicroBeads, one LS Column, and a MidiMACS Separator have been used. Cells were fluorescently stained with CD34-FITC, CD38-APC, CD45-VioBlue®
, Labeling Check Reagent‑APC, and Propidium Iodide Solution and analyzed by flow cytometry using the MACSQuant. Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
Before CD38 depletion
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