Find the products and resources you are looking for!
Miltenyi Biotec distribution:
Mouse ES cell cultures containing 14% early differentiated cells (A), as they can occur during feeder-free cultivation, were separated using the Pluripotent Stem Cell Isolation Kit, an LD Column, and a MidiMACS™ Separator. Differentiated cells were completely removed resulting in a purity of approx. 99% pluripotent cells (B). Flow cytometric analysis using the MACSQuant® Analyzer. Debris and dead cells were excluded based on scatter signals and propidium iodide fluorescence.
Phase contrast pictures of fixed feeder-free mouse embryonic stem cell cultures one day after plating without (A) and with separation (B) indicate efficient removal of differentiated cells.
Copyright © 2020 Miltenyi Biotec and/or its affiliates. All rights reserved.