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Neonatal cardiomyocytes were isolated from P2 CD-1®
hearts using the Neonatal Heart Dissociation Kit, mouse and rat, the Neonatal Cardiomyocyte Isolation Kit, mouse, an MS Column, and a MiniMACS™
Separator. Subsequently, cells were fixed and stained using the Inside Stain Kit (# 130-090-477) and a FITC-conjugated antibody specific for cardiomyocytes (anti-α-actinin). Cells were analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris were excluded from the analysis based on scatter signals.
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