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Cardiac endothelial cells were isolated from P1 CD-1 mouse hearts by using the Neonatal Cardiac Endothelial Cell Isolation Kit, one LD Column with MidiMACS™
Separator, and one MS Column with MiniMACS™ Separator. The cells were fluorescently stained with CD90.2-APC (# 130-091-790) and CD31-PE (# 130-097-422) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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