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Untouched switched memory B cells were isolated from human PBMCs using the Switched Memory B Cell Isolation Kit, an LS Column, and a MidiMACS™ Separator. Cells were fluorescently stained with CD19-FITC, Anti-IgD-PE, Anti-IgM-PE, Anti-IgA-APC, and Anti-IgG-APC and analyzed by flow cytometry. The cells are gated on CD19+
PBMCs before separation
Enriched switched memory B cells
Other B cells
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