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cells were isolated from PBMCs (that were previously prepared from B-CLL blood samples) using the Anti-ROR1 MicroBead Kit, an LS Column, and a MidiMACS™
Separator. Cells were fluorescently stained with CD19-APC (# 130-091-248) and analyzed by flow cytometry using the MACSQuant®
Analyzer. Cell debris and dead cells were excluded from the analysis based on scatter signals and propidium iodide fluorescence.
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